■Lumen 200: Superior Fluorescence Illumination

■ Powerful 200W Illumination A 200W metal-halide lamp gives a similar spectral output to older mercury vapour lamps and the metal-halide has over seven times longer lamp life.

 ■ Long Life Lamp - Up to 2000 Hours
The metal-halide lamp is safe for use for up to 2000 hours depending on operational factors. Internal electronics monitor the number of hours used and warn the user through both a visual and audible alarm, when a lamp replacement is necessary.

 ■ Easy Lamp Replacement
Unlike mercury vapour lamps the lamps used in the Lumen 200 system require no precision arc alignment for them to work properly. Lamps are pre-centred, so changes take less than one minute.

■ Stable Light Output Over 2000 Hour Lifetime
The Lumen 200 is engineered specifically to keep the 200W lamp at optimum temperature. This delivers unrivalled stability both in long term and short term output.

■ Low Heat Transfer
By mounting the light source remotely from the microscope stand the amount of heat transferred is significantly lower. This aids stability and helps reduce driftdue to thermal expansion in long term time lapse experiments.

■ Lamp Lifetime
The specially designed lamp has a linear degradation over 2000 hours of use, this provides a stable light source for quantitative and qualitative analysis

■ Manual Shutter & Attenuation
The Lumen 200 features a manually operated shutter mechanism that can be partially colsed to attenuate illumination intensity protecting valuable samples from photo-bleaching.

■Lumen Options and their Spectral Output

The three available options for the Lumen are:
L200 Standard microscopy	L210 Tuned for Cy5 and Cy5.5	L220 Extended in UVand IR for Cy7

■Lumen 200Pro

Advanced Fluorescence Illumination with Integral Automated Light Attenuation and High Speed Filter Wheel

The Lumen 200Pro features a built in, six position filter wheel for automated control of excitation wavelength. Lumen 200Pro also includes a variable aperture motorised shutter allowing computer controlled shuttering and light attenuation. The unit is driven by the Prior range of ProScanTMll, OptiscanTMll or PriorPCITMII advanced motor controllers, allowing it to be combined with the highest performance scanning stages and focusing devices avilable. By removing the filter wheel and shutter from direct contact with the microscope stand problems of vibration, heat transfer and long settling times are practically eliminated.

■ Shutters & Attenuation
The simple, robust and efficient design of the shuttering light attenuator provides both high speed and reliabiliyt. The less than 20 ms opening and closing speed will help to extend live cell studies by minimising the toxicity associated with photo-stepper motor driven shutter is designed to provide years of reliable uninterrupted serice.

■ High Speed Motorised Filter Wheel
The Lumen 200PRO features a built in, six position filter wheel compatible with all standard 25mm diameter filters for automated control of excitation wavelength. The unit is driven by the Prior range of ProScanTMll, OptiScanTMll and Prior PCI II advanced motor controllers allowing it to be combined with the higest performance scanning stages and focusing devices available. High speed filter changing with adjacent positions changed in 60ms with the ProScanTMll controller.

■ Software Compatibility
Controlled by either the PriorPCITMII, PriorPCITMII or PriorPCl ll makes adding the Lumen 200PRO to existing code controlling Prior Scientific automation equipment quick and easy. The Lumen 200PRO's internal filter wheel responds to existing standard filter wheel commands whether throuth the DLL or via ASCII commands, and light intensity is controlled with one additional command.

■Lumen 200Pro For Automated Applications

Uneven Illumination	Lumen 200 Illumination

One of the common problems with the conventional fluorescence microscope light source is optiming and maintaining its proper adjustment to provide even illumination over the whole fileld of view. Uneven illumination becomes much more of a problem when using digital imaging. This's because contrast stretching used to bring out the signals from the background also enhances uneven illumination across the image. Note how the chromosomes in the images taken using a conventional lamp become darker around the periphery of the image, the red signal on the left is nearly lost altogether.